伏見研究室　Fushimi Lab　

伏見研究室　Fushimi Lab　TOP

原著論文　Articles

査読あり、# 共同筆頭著者、* 共同責任著者

  1. Keiji Fushimi#, Yusuke Nakai#, Akiko Nishi, Ryo Suzuki, Masahiro Ikegami, Risa Nimura, Taichi Tomono, Ryota Hidese, Hisashi Yasueda, Yusuke Tagawa and Tomohisa Hasunuma: Development of the autonomous lab system to support biotechnology research, Sci. Rep., 15(1), 6648, 2025.
  2. Hiroki Hoshino, Keita Miyake, Keiji Fushimi, and Rei Narikawa: Red/green cyanobacteriochromes acquire isomerization from phycocyanobilin to phycoviolobilin, Protein Sci., 33(8), e5132, 2024.
  3. Mana Fukazawa, Keita Miyake, Hiroki Hoshino, Keiji Fushimi, and Rei Narikawa: Phytochromobilin binding and specific amino acid residues near the chromophore contribute to orange lght perception by the dualchrome phytochrome region, Plant Cell Physiol., pcae077, 2024.
  4. Yuka Takeda, Itsuki Ohtsu, Takahisa Suzuki, Yusuke Nakasone, Keiji Fushimi, Masahiko Ikeuchi, Masahide Terazima, Hideo Dohra and Rei Narikawa: Conformational change in an engineered biliverdin-binding cyanobacteriochrome during the photoconversion process, Arch. Biochem. Biophys., 745, 109715, 2023.
  5. Takahisa Suzuki*, Masataka Yoshimura, Hiroki Hoshino, Keiji Fushimi, Munehito Arai and Rei Narikawa*: Introduction of reversible cysteine ligation ability to the biliverdin-binding cyanobacteriochrome photoreceptor, FEBS J., 290(20), 4999-5015, 2023.
  6. Hirohide Takemura, Jae-Hoon Choi, Keiji Fushimi, Rei Narikawa, Jing Wu, Mitsuru Kondo, David C Nelson, Tomohiro Suzuki, Hitoshi Ouchi, Makoto Inai, Hirofumi Hirai and Hirokazu Kawagishi: Role of hypoxanthine-guanine phosphoribosyltransferase in the metabolism of fairy chemicals in rice, Org. Biomol. Chem., 21(12), 2556-2561, 2023.
  7. Yuto Kuwasaki, Kazushi Suzuki, Gaigai Yu, Shota Yamamoto, Takahiro Otabe, Yuki Kakihara, Michiru Nishiwaki, Keita Miyake, Keiji Fushimi, Ramsey Bekdash, Yoshihiro Shimizu, Rei Narikawa, Takahiro Nakajima, Masayuki Yazawa and Moritoshi Sato: MagRed: A red light–responsive photoswitch for deep tissue optogenetics, Nat. Biotechnol., 40(11), 1672-1679, 2022.
  8. Yuko Makita#, Shigekatu Suzuki#, Keiji Fushimi#, Setsuko Shimada#, Aya Suehisa, Manami Hirata, Tomoko Kuriyama, Yukio Kurihara, Hidefumi Hamasaki, Emiko Okubo-Kurihara, Kazutoshi Yoshitake, Tsuyoshi Watanabe, Masaaki Sakuta, Takashi Gojobori, Tomoko Sakami, Rei Narikawa, Haruyo Yamaguchi, Masanobu Kawachi and Minami Matsui: Identification of a dual orange/far-red and blue light photoreceptor from an oceanic green picoplanktonin, Nat. Commun., 21(1), 3593, 2021.
  9. Sean R. Tachibana, Longteng Tang, Liangdong Zhu, Yuka Takeda, Keiji Fushimi, Yoshibumi Ueda, Takahiro Nakajima, Yuto Kuwasaki, Moritoshi Sato, Rei Narikawa, and Chong Fang: An engineered biliverdin-compatible cyanobacteriochrome enables a unique ultrafast reversible photoswitching pathway, Int. J. Mol. Sci., 22(10), 5252, 2021.
  10. Keiji Fushimi* and Rei Narikawa*: Unusual ring D fixation by three crucial residues promotes phycoviolobilin formation in the DXCF-type cyanobacteriochrome without the second Cys, Biochem. J., 478(5), 1043-1059, 2021.
  11. Sean R. Tachibana, Longteng Tang, Cheng Chen, Liangdong Zhu, Yuka Takeda, Keiji Fushimi, Travis K. Seevers, Rei Narikawa, Moritoshi Sato and Chong Fang: Transient electronic and vibrational signatures during reversiblephotoswitching of a cyanobacteriochrome photoreceptor, Spectrochim. Acta A Mol. Biomol. Spectrosc., 250, 119379, 2021.
  12. Keiji Fushimi#, Hiroki Hoshino#, Naeko Shinozaki-Narikawa, Yuto Kuwasaki, Keita Miyake, Takahiro Nakajima, Moritoshi Sato, Fumi Kano and Rei Narikawa: The cruciality of single amino acid replacement for the spectral tuning of biliverdin-binding cyanobacteriochromes, Int. J. Mol. Sci., 21(17), 6278, 2020.
  13. Keiji Fushimi, Takumi Matsunaga and Rei Narikawa: A photoproduct of DXCF cyanobacteriochromes without reversible Cys ligation is destabilized by rotating ring twist of the chromophore, Photochem. Photobiol. Sci., 19(10), 1289-1299, 2020.
  14. Keiji Fushimi*, Masumi Hasegawa, Takeru Ito, Nathan C. Rockwell, Gen Enomoto, Ni-Ni-Win, J. Clark Lagarias, Masahiko Ikeuchi and Rei Narikawa*: Evolution-inspired design of multicolored photoswitches from a single cyanobacteriochrome scaffold, Proc. Natl. Acad. Sci. USA, 117(27), 15573-15580, 2020.
  15. Keita Miyake, Keiji Fushimi, Tomonori Kashimoto, Kaisei Maeda, Ni-Ni-Win, Hiroyuki Kimura, Masakazu Sugishima, Masahiko Ikeuchi and Rei Narikawa: Functional diversification of two bilin reductases for light perception and harvesting in unique cyanobacterium Acaryochloris marina MBIC 11017, FEBS Journal, 287(18), 4016-4031, 2020.
  16. Yuto Kuwasaki, Keita Miyake, Keiji Fushimi, Yuka Takeda, Yoshibumi Ueda, Takahiro Nakajima, Masahiko Ikeuchi, Moritoshi Sato and Rei Narikawa: Protein engineering of dual-Cys cyanobacteriochrome AM1_1186g2 for biliverdin incorporation and far-red/blue reversible photoconversion, Int. J. Mol. Sci., 20(12), 2935, 2019.
  17. Keiji Fushimi, Takatsugu Miyazaki, Yuto Kuwasaki, Takahiro Nakajima, Tatsuro Yamamoto, Kazushi Suzuki, Yoshibumi Ueda, Keita Miyake, Yuka Takeda, Jae-Hoon Choi, Hirokazu Kawagishi, Enoch Y. Park, Masahiko Ikeuchi, Moritoshi Sato and Rei Narikawa: Rational conversion of chromophore selectivity of cyanobacteriochromes to accept mammalian intrinsic biliverdin, Proc. Natl. Acad. Sci. USA, 116(17), 8301-8309, 2019.
  18. 崔 宰熏、呉 静、伏見 圭司、平井 浩文、河岸 洋和：シバ Agrostis stolonifera 由来のコムラサキシメジ菌糸体に対する生育抑制物質、日本きのこ学会誌、25(4), 141-144, 2018.
  19. Masumi Hasegawa, Keiji Fushimi, Keita Miyake, Takahiro Nakajima, Yuki Oikawa, Gen Enomoto, Moritoshi Sato, Masahiko Ikeuchi and Rei Narikawa: Molecular characterization of DXCF cyanobacteriochromes from the cyanobacterium Acaryochloris marina identifies a blue-light power sensor, J. Biol. Chem., 293(5), 1713-1727, 2018.
  20. Keiji Fushimi, Masahiko Ikeuchi and Rei Narikawa: The expanded red/green cyanobacteriochrome lineage: An evolutionary hot spot, Photochem. Photobiol., 93(3), 903-906, 2017.
  21. Keiji Fushimi, Gen Enomoto, Masahiko Ikeuchi and Rei Narikawa: Distinctive properties of dark reversion kinetics between two red/green-type cyanobacteriochromes and their application in the photoregulation of cAMP synthesis, Photochem. Photobiol., 93(3), 681-691, 2017.
  22. Keiji Fushimi, Nathan C. Rockwell, Gen Enomoto, Ni-Ni-Win, Shelley S. Martin, Fei Gan, Donald A. Bryant, Masahiko Ikeuchi, J. Clark Lagarias and Rei Narikawa: Cyanobacteriochrome photoreceptors lacking the canonical Cys residue, Biochemistry, 55(50), 6981-6995, 2016.
  23. Keiji Fushimi, Takahiro Nakajima, Yuki Aono, Tatsuro Yamamoto, Ni-Ni-Win, Masahiko Ikeuchi, Moritoshi Sato and Rei Narikawa: Photoconversion and fluorescence properties of a red/green-type cyanobacteriochrome AM1_C0023g2 that binds not only phycocyanobilin but also biliverdin, Front. Microbiol., 7, 588, 2016.
  24. Rei Narikawa, Keiji Fushimi, Ni-Ni-Win and Masahiko Ikeuchi: Red-shifted red/green-type cyanobacteriochrome AM1_1870g3 from the chlorophyll d-bearing cyanobacterium Acaryochloris marina, Biochem. Biophys. Res. Commun., 461(2), 390-395, 2015.
  25. Tatsuo Asai, Jae-Hoon Choi, Takashi Ikka, Keiji Fushimi, Nobuo Abe, Hidekazu Tanaka, Yasuhiro Yamakawa, Hajime Kobori, Yoshikazu Kiriiwa, Reiko Motohashi, Vipin Kumar Deo, Tomohiro Asakawa, Toshiyuki Kan, Akio Morita and Hirokazu Kawagishi: Effect of 2-azahypoxanthine (AHX) produced by the fairy-ring-forming fungus on the growth and the grain yield of rice, Jpn. Agric. Res. Quart., 49(1), 45-49, 2015.
  26. Rei Narikawa, Takahiro Nakajima, Yuki Aono, Keiji Fushimi, Gen Enomoto, Ni-Ni-Win, Shigeru Itoh, Moritoshi Sato and Masahiko Ikeuchi: A biliverdin-binding cyanobacteriochrome from the chlorophyll d-bearing cyanobacterium Acaryochloris marina, Sci. Rep., 5, 7950, 2015.
  27. Rei Narikawa, Gen Enomoto, Ni-Ni-Win, Keiji Fushimi and Masahiko Ikeuchi: A new type of dual-Cys cyanobacteriochrome GAF domain found in cyanobacterium Acaryochloris marina, which has an unusual red/blue reversible photoconversion cycle, Biochemistry, 53(31), 5051-5059, 2014.
  28. Keiji Fushimi, Kota Anzai, Shinji Tokuyama, Yoshikazu Kiriiwa, Noriyuki Matsumoto, Atsushi Sekiya, Daisuke Hashizume, Kazuo Nagasawa, Hirofumi Hirai and Hirokazu Kawagishi: Agrocybynes A-E from the culture broth of Agrocybe praecox, Tetrahedron, 68(4), 1262-1265, 2012.
  29. Jing Wu, Keiji Fushimi, Shinji Tokuyama, Masato Ohno, Takaaki Miwa, Tomoyuki Koyama, Kazunaga Yazawa, Kaoru Nagai, Tetsuo Matsumoto, Hirofumi Hirai and Hirokazu Kawagishi: Functional-food constituents in the fruiting bodies of Stropharia rugosoannulata, Biosci. Biotechnol. Biochem., 75(8), 1631-1634, 2011.
  30. Keiji Fushimi, Madoka Horikawa, Kaori Suzuki, Atsushi Sekiya, Susumu Kanno, Susumu Shimura and Hirokazu Kawagishi: Applanatines A-E from the culture broth of Ganoderma applanatum, Tetrahedron, 66(48), 9332-9335, 2010.
  31. Jae-Hoon Choi, Keiji Fushimi, Nobuo Abe, Hidekazu Tanaka, Setsuko Maeda, Akio Morita, Miyuki Hara, Reiko Motohashi, Junichi Matsunaga, Yoko Eguchi, Naomi Ishigaki, Daisuke Hashizume, Hiroyuki Koshino and Hirokazu Kawagishi: Disclosure of the “Fairy” of fairy-ring-forming fungus Lepista sordida, ChemBioChem, 11(10), 1373-1377, 2010.
  32. Jae-Hoon Choi, Nobuo Abe, Hidekazu Tanaka, Keiji Fushimi, Yoshifumi Nishina, Akio Morita, Yoshikazu Kiriiwa, Reiko Motohashi, Daisuke Hashizume, Hiroyuki Koshino and Hirokazu Kawagishi: Plant-growth regulator, imidazole-4-carboxamide produced by fairy-ring forming fungus Lepista sordida, J. Agr. Food Chem., 58(18), 9956-9959, 2010.

総説論文　Reviews

  1. 伏見 圭司、成川 礼：シアノバクテリオクロムの進化的系譜から着想を得た多彩な光変換分子の合理的設計、光合成研究、30(2)、96-109、2020.
  2. 伏見 圭司、成川 礼：哺乳類内在性色素を結合するシアノバクテリオクロム、バイオサイエンスとインダストリー、77(6)、472-474、2019.
  3. 伏見 圭司、成川 礼：光を感知して色が変わるシアノバクテリオクロム、バイオサイエンスとインダストリー、77(6)、436-437、2019.
  4. Keiji Fushimi and Rei Narikawa: Cyanobacteriochromes: Photoreceptors covering the entire UV-to-visible spectrum, Curr. Opin. Struct. Biol., 57, 39-46, 2019.
  5. 伏見 圭司、成川 礼：光合成生物における開環テトラピロール結合型光受容体、生物物理、58(6)、303-307、2018.
  6. 伏見 圭司、成川 礼：遠赤色光を吸収して光変換しつつ蛍光を発するタンパク質の発見と応用利用、ケミカルエンジニアリング、61(7)、13-17、2016.

著書　Books

  1. Keiji Fushimi and Rei Narikawa: Phytochromes and cyanobacteriochromes: Photoreceptor Molecules Incorporating a Linear Tetrapyrrole Chromophore, Optogenetics: Light-sensing Proteins and Their Applications in Neuroscience and Beyond (Second Edition), Springer, Chapter 10, 167-187, 2021/01/06.
  2. Keita Miyake, Keiji Fushimi and Rei Narikawa: The Diversity of Cyanobacterial Photoresponsive Systems, Green Science and Technology, CRC Press, Chapter 8, 116-126, 2019/11/12.

研究費　Grants

  1. 伏見 圭司、公益財団法⼈ 発酵研究所、キノコの⼦実体発⽣に関わる⾚⾊光感知型光受容体の分⼦機能解明（代表）、3,000,000 円（総額）、2025 – 2026 年度
  2. 伏見 圭司、公益財団法人 内藤科学技術振興財団、カゼインから生成される子実体発生誘導物質の発見（代表）、1,000,000 円（総額）、2025 年度
  3. 伏見 圭司、ひょうご科学技術協会 学術研究助成事業、酵素反応の新しい可能性を見出す分子基盤の構築（代表）、1,000,000 円（総額）、2024 – 2025 年度（諸事情により辞退）
  4. 伏見 圭司、日本学術振興会 科学研究費助成事業（科研費）基盤研究（C）、カビと光の関係–真菌類の光感知が導く分化誘導制御機構の解明に向けた基盤構築（代表、21K05339）、4,160,000 円（総額）、2021 – 2023 年度